| 质粒名称: | pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117168 |
| 存储实验室: | Steven Jacobsen |
|
载体骨架基本信息 |
|
| 载体名称: | pEG302 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR，合成生物学 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS,22aa SunTag NtDRMcd (no NLS) g4+g10+g18 |
| 物种来源: | -- |
| 插入大小: | 17388 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA) was a gift from Steven Jacobsen (Addgene plasmid # 117168 ; http://n2t.net/addgene:117168 ; RRID:Addgene_117168) |
| 参考文献部分: | Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722 |
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
 g4+g10+g18 (FWA)质粒图谱.png)
载体简介
载体序列
LOCUS pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA) 27421 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the
FWA locus with three guide RNAs.
ACCESSION .
VERSION .
KEYWORDS pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 27421)
AUTHORS Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
TITLE Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9
SunTag systems.
JOURNAL Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
PUBMED 30760722
REFERENCE 2 (bases 1 to 27421)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..27421
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 4616..4640
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4659..4714
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 4728..8831
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8835..8861
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8880..8900
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8907..8927
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8937..8957
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 9021..9077
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9144..9200
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9267..9323
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9390..9446
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9513..9569
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9636..9692
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9759..9815
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9882..9938
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 10005..10061
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 10128..10184
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
terminator 10283..10990
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(11023..11041)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(11024..11041)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(11065..11082)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(11065..11081)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11074..11096)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 11284..11308
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 12609..13238
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 13667..14740
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 14806..15000
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(15236..15258)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 15344..15484
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 15399..15418
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(15499..15516)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(15670..16258)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(15750..15769)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(17683..18477)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 18902..18926
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 19016..19190
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(19231..20256)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(20323..20999)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(20366..20386)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
misc_RNA complement(21084..21159)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(21641..21716)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(22203..22278)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(23029..23079)
/codon_start=1
/product="nuclear localization signal from the human T-cell
leukemia virus type 1 (HTLV-1) Rex protein"
/label=Rex NLS
/translation="PKTRRRPRRSQRKRPPT"
CDS complement(23107..23268)
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS complement(24415..25125)
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(24455..24475)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 25070..25098
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
CDS complement(25216..25242)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
ORIGIN
1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
541 ttaaaagctc tgttgagcta gactcatatg catccctagg aaacaagttg taatgagttg
601 ctggcctctc ttgggattgt tcatcaaagt agctgtccct acacaccaca acagaatggt
661 gttgtggaga gaaagcacag acacatcctt gagatggcaa gggcacttaa gtttcagagt
721 ggtgtaccta ccaggttttg gggagactgt gtcaagactg ttgtatacct gatcaataga
781 ctccctactc caatcttaca aggcaaatgt ccatatgaac tactctatca gaaacatgcc
841 aagcttgatc acttgagagt gtttgggtgt cttgcttttg cagcaacact gcccaaaggt
901 gacaaacttg cacctagagc caagcgaacc atcttcattg gttattctga gactcaaaag
961 ggttacaggt tgtatgactt ggataataag gtgatcatag tgagcaggga tgtagttttc
1021 agagagtttc agttcccttt caaagagtcc atctcccatg aacctgatat gttcactcga
1081 gcaggttcac ttagctctga agatacagct ttgcagctag tgtataatga tatttttcct
1141 tccagctttc atactgatga tgatgatact ccagttctta ttcctgacat tgtagaggcc
1201 aatgaggata tcactttggg gactcattct acccaaaatc atatcacttc agctgatgct
1261 gcaccttcag gccacctgca tgctcctgca gagtcagttg agtcagctga gcctgaccta
1321 gcaaatgctg aatttgagca ccatactgct gtagctgatc cctctctagt tccagcaaac
1381 ctgcacacta gacctaaacg caatgcaggt cctcccatct ggctcaagga ctttgtgaca
1441 ctgaacaaag gctctaggga tgttccatac cctatagcca actatgtttc ctatgatcac
1501 ttgcctgttc actatcaagc ttatttgagt gctttctcca cttatactga acctaaatcc
1561 ttcaaagaag cagctcaaga tgagaaatgg atggaggcca tgtcccttga gatacaggct
1621 cttgaggata ataacacctg ggagattgtc cctttacccc ctggtaaaca gcctataggg
1681 tccaaatggg tgtacaaaat taaatacaaa gctaatggtg aagttgacag gtttaaggca
1741 aggctagtgg ccaagggata cactcagcaa gaaggccttg actaccatga aactttttct
1801 ccagtggcca aaatggtcac tgtaaaagct gtcatatctg ttgctgcttc caagggctgg
1861 ttccttttcc agatggatgt caacaatgct tttctacaag gtgacctcat ggaagaagtt
1921 tatatgtctc tgcctcaggg ttttcacagc caaggggaga ccaaagtgtg caggctgttg
1981 aatccctcta tggtttaaag caagcatcaa ggcagtggaa catcaagctt accactgtcc
2041 ttatgcaggc tggttttatg caaagtgctt atgatcactc cttgtttacc aaaagagagg
2101 ggactgacct tgtcataatc ctgatttatg tggatgatct gttaataaca ggcagcagca
2161 atgttttgat ttcagaagca aaggcaaccc tgcatcagca tttcaagatg aaagatttgg
2221 gagaactaaa atacttctta ggcattgagg tgctgagatc agaaaaggga atcctactga
2281 accagaggaa gtatgcactg gaactgatat caggtgttgg tctgggaggc tgcagaccag
2341 tgtcaacacc aatggaacag aaccaaaggc tgactactgt agaatatgac aagcacttag
2401 gaaagactga tgatgcagag ttagaggatg tgggttccta tcagagactg gtgggaaagc
2461 ttctctactt gacaatcaca aggccagata tcagctttgc agtgcaggtg ctgagtcaat
2521 tcatgcagca acccaaacag tcacacctgg aagcagcatt gagggttgtg aagtacataa
2581 aaggttctcc aggtgttagg aacgcggccg catgcatatg agtctagctc aacagagctt
2641 ttaacccaaa ttggtacaat agaatacaac tttagatcat aattctcaaa agaaagagat
2701 tccttagcta ttctatctgc cactccattt ccttctcggc ttgtatgcac aagcataaaa
2761 tcctcaaact tgctaagtag atactttatg tcttggataa ttggattgag acttgacaag
2821 cataactttc atgtaaccaa agacacaagt tgctgagaat ccacctcaaa aatgatcttc
2881 ctataattga atcgggataa tgacagcaca gcccatctaa gagcctccac ttctacttcc
2941 agcacgcttc ttacttttac cacagctctt gcacctaacc ataacacctt ccctgtatga
3001 tcgcgaagca cccaccctaa gccacatttt aatccttctg ttggccatgc cccatcaaag
3061 ttgcacttaa cccaagattg tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt
3121 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
3181 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
3241 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
3301 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
3361 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
3421 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
3481 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
3541 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
3601 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaattaaa
3661 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
3721 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
3781 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
3841 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
3901 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaga
3961 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
4021 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
4081 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
4141 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
4201 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
4261 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
4321 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
4381 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
4441 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
4501 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
4561 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagggat catcaacaag
4621 tttgtacaaa aaagcaggct ctttaaagta tttttacaac aattaccaac aacaacaaac
4681 aacaaacaac attacaatta ctatttacaa ttacaaaaaa agttaacatg gacaagaagt
4741 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
4801 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
4861 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
4921 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
4981 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
5041 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
5101 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
5161 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
5221 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
5281 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
5341 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
5401 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
5461 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
5521 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
5581 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
5641 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
5701 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
5761 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
5821 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
5881 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
5941 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
6001 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
6061 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
6121 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
6181 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
6241 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
6301 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
6361 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
6421 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
6481 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
6541 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
6601 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
6661 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
6721 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
6781 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
6841 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
6901 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
6961 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
7021 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
7081 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
7141 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
7201 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
7261 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
7321 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
7381 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
7441 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
7501 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
7561 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
7621 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
7681 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
7741 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
7801 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
7861 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
7921 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
7981 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
8041 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
8101 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
8161 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
8221 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
8281 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
8341 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
8401 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
8461 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
8521 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
8581 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
8641 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
8701 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
8761 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
8821 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
8881 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggaa gatgctccaa
8941 agaagaagag aaaggtcgac ggcattggta gtgggagcaa cggcagcagc ggatccaacg
9001 gtccgactga cgccgcggaa gaagaacttt tgagcaagaa ttatcatctt gagaacgaag
9061 tggctcgtct taagaaaggt tctggcagtg gaggttctgg ctccggatct ggtggttcgg
9121 gctcaggcgg gtccggatca ggcgaagaac tgctttcaaa gaattaccac ctggaaaatg
9181 aggtagctag actgaaaaag gggagcggaa gtgggggctc cgggtcgggc tcagggggct
9241 ccggttcggg aggctcaggg tcgggggagg agttgctgag caaaaattat catttggaga
9301 acgaagtagc acgactaaag aaagggtccg gatcgggtgg ttcaggatct ggatccggag
9361 gatcagggtc cggtgggtcg ggctcaggag aggagttact ctcgaaaaat tatcatctcg
9421 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg aggatctggg tcggggtcag
9481 gcgggtctgg atctggggga tctggatctg gtgaagagct attatctaaa aactaccacc
9541 tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgggtcg ggatctggct
9601 ctggtggctc aggctcggga ggttcaggtt ccggggaaga gctactatcc aagaattatc
9661 atcttgagaa cgaggtagcg cgtttgaaga agggttccgg ctcaggagga tctgggtcag
9721 gatcgggggg ttccgggtca ggcgggtccg ggtcaggcga ggaactgctc tcgaagaact
9781 atcatcttga aaatgaggtc gctcgattaa aaaagggatc gggcagtggt gggtccggct
9841 ccggttccgg aggatcggga tctgggggct cgggatccgg ggaggaacta ctttcaaaga
9901 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtggctcag
9961 gttcgggatc aggtgggtca ggctccggtg gttcaggttc gggagaagaa ttactgagta
10021 aaaattatca tctggaaaat gaggtagcga gactaaaaaa ggggagtggt tctggcggtt
10081 cgggatctgg ctctgggggc tctgggtcgg gagggtctgg gtctggcgag gaattgctat
10141 cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggcg
10201 ggtccgggtc agggtcggga ggttccggat ccgggggatc aggctcaggg taacccgggg
10261 gcgcgccatg ataactagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
10321 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
10381 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
10441 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
10501 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
10561 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
10621 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
10681 ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt
10741 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
10801 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
10861 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
10921 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
10981 ggctcagcag gaccggcatg caagctagct tactagtgat attctatagt gtcacctaaa
11041 tctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
11101 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
11161 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
11221 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
11281 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
11341 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
11401 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
11461 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
11521 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
11581 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
11641 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
11701 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
11761 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
11821 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
11881 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
11941 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
12001 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
12061 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
12121 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
12181 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
12241 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
12301 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
12361 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
12421 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
12481 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
12541 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
12601 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
12661 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
12721 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
12781 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
12841 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
12901 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
12961 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
13021 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
13081 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
13141 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
13201 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
13261 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
13321 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
13381 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
13441 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
13501 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
13561 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
13621 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
13681 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
13741 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
13801 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
13861 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
13921 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
13981 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
14041 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
14101 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
14161 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
14221 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
14281 gcgtacaaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
14341 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
14401 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
14461 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
14521 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
14581 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
14641 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
14701 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
14761 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
14821 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
14881 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
14941 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
15001 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
15061 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
15121 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
15181 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
15241 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
15301 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
15361 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
15421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
15481 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
15541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
15601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
15661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
15721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
15781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
15841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
15901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
15961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
16021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
16081 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
16141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
16201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
16261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
16321 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
16381 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
16441 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
16501 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
16561 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
16621 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
16681 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
16741 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
16801 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
16861 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
16921 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
16981 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
17041 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
17101 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
17161 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
17221 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
17281 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
17341 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
17401 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
17461 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
17521 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
17581 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
17641 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
17701 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
17761 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
17821 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
17881 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
17941 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
18001 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
18061 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
18121 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
18181 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
18241 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
18301 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
18361 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
18421 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
18481 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
18541 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
18601 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
18661 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
18721 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
18781 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
18841 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
18901 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
18961 ggacgttttt aatgtactga attaacgccg aattaattcg agctctaatt cgggggatct
19021 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
19081 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
19141 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
19201 tcgatcgaca gatcccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
19261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
19321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
19381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
19441 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
19501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
19561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
19621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
19681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
19741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
19801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
19861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
19921 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgaacggcg
19981 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
20041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
20101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
20161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
20221 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cggatctgcg
20281 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
20341 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
20401 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
20461 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
20521 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
20581 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
20641 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
20701 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca
20761 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
20821 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
20881 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
20941 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
21001 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
21061 gctccaccat ggtaccaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
21121 actagcctta ttttaacttg ctatttctag ctctaaaacc tctttatccc attcaacatc
21181 aatcactact tcgactctag ctgtatataa actcagcttc gttttcttat ctaagcgatg
21241 tgggactttt gaagattgtt ttcaacttaa atgggcctat ataagaaata ctattgttct
21301 ttcccatata aatgggcctg cttctcttct ttcagattcc caggggcctt ttgaagatta
21361 tcttcatatc ttaagaatga agatgtttta ttcaatcaaa ttcttgaagg ttcgatgcct
21421 aatcattcta atcctgggac aaactatgaa acaagataca aaaactccga atggaaagtt
21481 aaaaagaaga aaacgaaagc tacggttcaa gaaaatgtaa gctgataaac aaaaaaaaac
21541 tgtatgaacg aagaagaaga aaaaaagcta agaagaaatg atgtattgtg cggaaggcaa
21601 gtcgagtttc cgttgttcaa cgaaagcttg tttaaaaaaa gcaccgactc ggtgccactt
21661 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaacttca
21721 acattcatac gagcgccaat cactacttcg actctagctg tatataaact cagcttcgtt
21781 ttcttatcta agcgatgtgg gacttttgaa gattgttttc aacttaaatg ggcctatata
21841 agaaatacta ttgttctttc ccatataaat gggcctgctt ctcttctttc agattcccag
21901 gggccttttg aagattatct tcatatctta agaatgaaga tgttttattc aatcaaattc
21961 ttgaaggttc gatgcctaat cattctaatc ctgggacaaa ctatgaaaca agatacaaaa
22021 actccgaatg gaaagttaaa aagaagaaaa cgaaagctac ggttcaagaa aatgtaagct
22081 gataaacaaa aaaaaactgt atgaacgaag aagaagaaaa aaagctaaga agaaatgatg
22141 tattgtgcgg aaggcaagtc gagtttccgt tgttcaacga aagctttcct aaaccaaaaa
22201 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
22261 tatttctagc tctaaaacaa gcccatacat ctttccgtca atcactactt cgactctagc
22321 tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg aagattgttt
22381 tcaacttaaa tgggcctata taagaaatac tattgttctt tcccatataa atgggcctgc
22441 ttctcttctt tcagattccc aggggccttt tgaagattat cttcatatct taagaatgaa
22501 gatgttttat tcaatcaaat tcttgaaggt tcgatgccta atcattctaa tcctgggaca
22561 aactatgaaa caagatacaa aaactccgaa tggaaagtta aaaagaagaa aacgaaagct
22621 acggttcaag aaaatgtaag ctgataaaca aaaaaaaact gtatgaacga agaagaagaa
22681 aaaaagctaa gaagaaatga tgtattgtgc ggaaggcaag tcgagtttcc gttgttcaac
22741 gaaagcttcg taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
22801 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
22861 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
22921 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
22981 aaatgtttga acgatcgggg aaattcgagc tcaaacttac ggccatggcg taggcggcct
23041 tttccgttgt gatcttcgcg gtctcctcct tgtttttgga ctagtaccac caccgctacc
23101 accaccttcg gttaccgtga aggttttggt agcgtcgtcg taggtccatt caccgtcaac
23161 accgttgtcg ttagcgtact gtttgaaaac tttttccgcg gtagcagcgt caacagcttc
23221 ggtggtggtt tcacctttca gggttttacc gttcaggata agcttgtact cttcggtccg
23281 agatcctcct cccgtacgcc ctccgccacc gccatgttta tgtctggaca ttatggactt
23341 gaccaagtcc aatatccgaa catagtcata aaatagggaa gactctttgc cttcaagccc
23401 atccctgctc actctgttac tgcctgcaag gttattgcac gggcttccac caattaccaa
23461 atcaaatcct ccaaatgact ctatcagttg ctccaagcgg tctccattca gctgctgcac
23521 atcgttaaaa tgtataaggt tccctctctg attagtttgc tcccaccagc ttctcacaat
23581 atccctgttg acttcagatt tttccacaga aaccactgtg tttagtggaa taccaagacg
23641 ataaagagca acttcaccac cgccaattcc agagaagagt gataagacat tgataccacc
23701 gggaaacaag tctttcaata ccgacaaatg gtatgccaca gtgtcaacct ggaatgaatt
23761 accaagcgac ttgtatctat cggtcctact tataccacct cccctagtat ggttctttgg
23821 aaatcccagt agcatttcaa cttcatcagg ctccaaagga gcaactttgt ttctcccaac
23881 ccacaccaaa ttccacttcc gacattgatc aagaacaaac ttctgaactc tcattggtgg
23941 ctcaccatca aagtcctcca cagctttcct aatcctattc gtcaattgtg cacttccaat
24001 ggctgtttgc aaacaattta actttgtccg tgggtcccaa gatggccacc atttcttcga
24061 taggggaagt gcctcatgaa tggtacgtgg ggcaagtgga aacaaaggaa atctattttc
24121 aaccggcaga ttatgaatat aacccctttt tcttgcagca gcacaaaaat atttggagtc
24181 gacaaactct ggctcaatat cgtacaaaaa tctagaaatt gtgtcccaca caccctttgg
24241 agctagagcc acattttcat agtagaaaaa cgggggtcca acagcttgct cgggaagagt
24301 tcttcgaacc attgctggaa gtggttcggt aggaacccca aacccaatca taggtttggg
24361 caaacgaatt gtctcccctc cgccaccgcc cgtacgaccg gtccgacctc cacctttgta
24421 gagctcatcc atgccatgtg taatcccagc agcagttaca aactcaagaa ggaccatgtg
24481 gtcacgcttt tcgttgggat ctttcgaaag gacagattgt gtcgacaggt aatggttgtc
24541 tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg
24601 aacggaacca tcttcaacgt tgtggcgaat tttgaagtta gctttgattc cattcttttg
24661 tttgtctgcc gtgatgtata cattgtgtga gttaaagttg tactcgagtt tgtgtccaag
24721 aatgtttcca tcttctttaa aatcaatacc ctttaactcg atacgattaa caagggtatc
24781 accttcaaac ttgacttcag cacgcgtctt gtaggtcccg tcatctttga aagatatagt
24841 gcgttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatgtg
24901 atccggataa cgggaaaagc attgaacacc ataggtcaga gtagtgacaa gtgttggcca
24961 cggaacaggt agttttccag tagtgcaaat aaatttaagg gtgagttttc cgtttgtagc
25021 atcaccttca ccctctccac ggacagaaaa tttgtgccca ttaacatcac catctaattc
25081 aacaagaatt gggacaactc cagtgaaaag ttcttctcct ttgctaccgc ttccacctcc
25141 acctggatcc aagcttccgc cgcctcctga accaccaccg ccactacctc ctcccccaga
25201 acctccgcct ccaccagcgt aatctggaac atcgtatggg tagctgctca cggtcaccag
25261 ggtgccctgg ccccagtagt cgaacaggcc ggtcacgcag tagtacaggg cggtgtcgtc
25321 gctgcgcacc ttgctcatct gcaggtacac ggtgttcttg ccgttgtcct tgctgatgat
25381 gaagcggtcc ttcagggcgc tgttgtagtc ggtgatgccg tcgccccaga tcacgccgat
25441 ccactccagg ccgcggccgg gggcctggcg cacccagttc acgccgtagt cggtcaggct
25501 gaagccgctc acggcgcagc tcagcttcag gctgccgccg ggctgcacca ggccgccgcc
25561 gctctccagc agcttcacct cgctgccgcc gccgctgctg ccgccgccgc cgctgccgcc
25621 gccgccgctg ccgccgccgc cgcgcttcag ctccaccttg gtgccctggc cgaacaccca
25681 gtggttgctg taccacaggg cgcagaagta ggtggcgaag tcctcgggct gcaggctgct
25741 gatggtcagg gtggccttgt cgccgatcag gctgccgctg aagcggctgg gcacgccggg
25801 ggcgcggttg ttggtgccgc cgatcaggcc cttgaacagc ttgccgggct tctcctgcac
25861 ccagctggcg tagttgctgg tggtcacggc gccggtgctg ctgcggcagg tgatggtcac
25921 gcggtcgccc acgctggcgc tcaggctgct ggggctctgg gtcatcacga tgtcggggcc
25981 catcagcgct aaaccctagg tgttaatcag aaaaactcag attaatcgac aaattcgatc
26041 gcacaaacta gaaactaaca ccagatctag atagaaatca caaatcgaag agtaattatt
26101 cgacaaaact caaattattt gaacaaatcg gatgatatct atgaaaccct aatcgagaat
26161 taagatgata tctaacgatc aaacccagaa aatcgtcttc gatctaagat taacagaatc
26221 taaaccaaag aacatatacg aaattgggat cgaacgaaaa caaaatcgaa gattttgaga
26281 gaataaggaa cacagaaatt taccttgatc acggtagaga gaattgagag aaagttttta
26341 agattttgag aaattgaaat ctgaattgtg aagaagaaga gctctttggg tattgtttta
26401 tagaagaaga agaagaaaag acgaggacga ctaggtcacg agaaagctaa ggcggtgaag
26461 caatagctaa taataaaatg acacgtgtat tgagcgttgt ttacacgcaa agttgttttt
26521 ggctaattgc cttattttta ggttgaggaa aagtatttgt gctttgagtt gataaacacg
26581 actcgtgtgt gccggctgca accactttga cgccgtttat tactgactcg tcgacaacca
26641 caatttctaa cggtcgtcat aagatccagc cgttgagatt taacgatcgt tacgatttat
26701 atttttttag cattatcgtt ttatttttta aatatacggt ggagctgaaa attggcaata
26761 attgaaccgt gggtcccact gcattgaagc gtatttcgta ttttctagaa ttcttcgtgc
26821 tttatttctt ttcctttttg tttttttttg ccatttatct aatgcaagtg ggcttataaa
26881 atcagtgaat ttcttggaaa agtaacttct ttatcgtata acatattgtg aaattatcca
26941 tttcttttaa ttttttagtg ttattggata tttttgtatg attattgatt tgcataggat
27001 aatgactttt gtatcaagtt ggtgaacaag tctcgttaaa aaaggcaagt ggtttggtga
27061 ctcgatttat tcttgttatt taattcatat atcaatggat cttatttggg gcctggtcca
27121 tatttaacac tcgtgttcag tccaatgacc aataatattt tttcattaat aacaatgtaa
27181 caagaatgat acacaaaaca ttctttgaat aagttcgcta tgaagaaggg aacttatccg
27241 gtcctagatc atcagttcat acaaacctcc atagagttca acatcttaaa caagaatatc
27301 ctgatctgaa gaatgtggag gctttagtcc cttggatact tgggaggctg tggaagaaca
27361 gaaacgagct ggtgcttaaa gggagggaat ttggaaccaa tgaggtatta gtaaggacac
27421 a
//
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